Overview
Grade UE06: £34,610 - £39,906 per annum
CMVM / SNCS / Centre for Discovery Brain Sciences
Fixed-Term: until 31 August 2026
Full-time: 35 hours per week
The Opportunity: You will contribute to supporting the research team with maintenance of human induced pluripotent stem cells (iPSCs), as well as differentiation into cortical organoids and oligodendrocytes. You will assist in characterising the effect of MAPT mutations on different cell types using various molecular biology techniques such as DNA/RNA isolation, western blotting and fluorescence microscopy, and aid with high resolution imaging techniques in human tissues. Training will be provided, as necessary. You must be able to work closely with other team members to develop and troubleshoot protocols, and will be responsible for your own daily organisation. This post is full-time (35 hours per week).
Responsibilities
* Maintain human induced pluripotent stem cells (iPSCs) and differentiate them into cortical organoids and oligodendrocytes.
* Assist in characterising the effect of MAPT mutations on different cell types using molecular biology techniques (DNA/RNA isolation, western blotting, fluorescence microscopy).
* Aid with high-resolution imaging techniques in human tissues.
* Receive training as necessary and work closely with the team to develop and troubleshoot protocols.
* Manage own daily organisation and contribute to a collaborative research environment.
Qualifications
* MSc degree in Neuroscience
* Prior experience in a laboratory environment
* Experience with iPSC culture, including neuronal and organoid differentiation
* Prior experience with viral transduction
* Experience with fluorescence microscopy platforms
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