Job Description
Main Purpose of the Job
This exciting project will combine functional genomics and bacteriophage (phage) biology to design combinations of phage to improve efficacy in killing of Enterotoxigenic Escherichia coli (ETEC) strains. This will form the basis for future development of a phage-based therapeutic for swine disease, in collaboration with an industry partner.
Your work will contribute to the understanding of the diversity of pathogenic E. coli strains affecting swine and identify variants of concern. You will isolate and genotypically characterise phages that kill swine-related pathogenic E. coli. You will also construct a transposon mutant library in E. coli to help the design of an efficacious phage cocktail against ETEC. You will receive supervision and training in microbiology, molecular biology and bioinformatics from Dr Hannah Pye, and will be integrated into two leading research groups focussed on bacteriophage biology and bacterial pathogen variation.
Key Relationships
You will work closely with Dr Evelien Adriaenssens and Prof. Rob Kingsley, who are both group leaders at the Quadram Institute Bioscience. The post holder will also work alongside other members of the Adriaenssens and Kingsley groups, who specialise in bacteriophages and bacterial pathogens, as well as other members of the Quadram Institute Bioscience. The Project Scientist will also be required to disseminate research findings to the industry partner.
Main Activities & Responsibilities
Functional genomics to study phage-bacteria interactionEstablishment of a phage biobank against pathogenic E. coli strainsGenotypic characterisation of E. coli phages, including whole genome sequencingAssessment of swine ETEC strain diversityReport writing and dissemination of project outcomesAs agreed with the line manager, any other duties commensurate with the nature of the post, for example, contributing to the work of Institute committees.
Person Profile
Education & Qualifications
RequirementBSc or equivalent in microbiology, or a related life sciences disciplineMSc or equivalent in microbiology, or a related life sciences discipline
Specialist Knowledge & Skills
RequirementMicrobiology techniques relating to bacterial cultureAbility to confidently conduct experiments independently once trained Demonstrated ability to maintain accurate and detailed records of laboratory experiments and results Knowledge of phage isolation techniquesKnowledge of microbial sequencing methods
Relevant Experience
RequirementLaboratory expertise in microbiology Laboratory experience in molecular biology techniques, such as PCR and production of electrocompetent cells Experience in bacterial/phage DNA extraction
Interpersonal & Communication Skills
RequirementAbility to work to deadlines Good communication skills, both written and verbalGood interpersonal skills, with the ability to work well as part of a team Demonstrated ability to work independently, using initiative and applying problem solving skills
Additional Requirements
RequirementAttention to detailPromotes equality and values diversityObserve best practices in data management and research integrityWillingness to embrace the expected values and behaviours of all staff at the Institute, ensuring it is a great place to workAble to present a positive image of self and the Institute, promoting both the international reputation and public engagement aims of the Institute Ability to maintain confidentiality and security of information where appropriate Willingness to work outside standard working hours when required