Role
Under the supervision of Dr Paul Fogg and Professor Fred Antson, the project will be to investigate the mechanism of DNA packaging by viruses and in particular the virus-like entities known as Gene Transfer Agents (GTAs). GTAs differ from true viruses in that they do not preferentially recognize and package their own DNA but instead target the bacterial host’s genome for dissemination. Comparison of viral and GTA structures and packaging mechanisms will lead to broad insights into virus replication. Differences in DNA specificity could also have important implications for the spread of genes, such as virulence factors or antimicrobial resistance genes (AMR), but could also be exploited for novel biotechnological applications. The research will necessitate production and purification of GTA particles and related bacteriophages, structure determination of proteins and protein-DNA assemblies by cryo-electron microscopy and single particle 3D-reconstruction. Structural studies will be complemented by molecular/genetic interrogation of the bacterial host and GTAs and possible mechanisms of DNA packaging will be analysed and probed with suitable biochemical assays.
Skills, Experience & Qualification needed
1. You should have, or about to obtain, a PhD in structural biology, biochemistry, microbiology/virology or related fields
2. Knowledge of the molecular biology & genetics of bacterial viruses
3. Experience of relevant techniques such as production and purification of bacteriophage or virus-like particles, protein purification, and structure determination by crystallography/Cryo-EM
4. Excellent communication skills demonstrated by publication of your research in high quality peer reviewed publications, presentations at conferences or other events, and engagement with public outreach
5. Experience of working both independently and part of a team
6. Collaborative ethos and positive attitude to colleagues and students
7. Attention to detail and commitment to high quality and ability to plan and prioritise own work to meet deadlines, and an ability to develop research objectives
8. Commitment to personal development and maintaining up to date knowledge and skills
Interview date: To be confirmed