Job Description
About the Company
Yellowstone Biosciences is a biotechnology spinout company located in Oxford, whose goal is to pioneer new cancer treatments using advanced T-cell therapies based on groundbreaking research from the MRC Weatherall Institute of Molecular Medicine at the University of Oxford.
About the Role
We are seeking two Scientists to work within our Protein Production team to provide essential hands-on support to accelerate our pipeline of novel therapeutics. A main aspect of one of these roles is to take responsibility for maintaining and optimising the mammalian expression pipeline; this is a key deliverable. Both roles require protein purification, characterisation and affinity measurements. There will be opportunity to gain broad experience across therapeutic protein design and characterisation in a fast-paced & collaborative biotech environment. The role is available as a fixed term contract (6 month) in the first instance.
Responsibilities
* Recombinant protein expression in mammalian systems
* Cell maintenance and expansion in preparation for transfection for the Protein Science team
* Transient transfection
* Stable cell line generation
* Protein purification (Bench and FPLC methods)
* Protein characterisation (SDS-PAGE, Western blotting and DSF)
* Protein binding assays (SPR, BLI, ELISA)
* Molecular biology Expression construct design and cloning
Qualifications
* PhD (or equivalent experience) in Biochemistry or a related subject
Required Skills
* Experience of mammalian cell culture and excellent aseptic technique (for mammalian expression role)
* Excellent knowledge of protein production techniques and workflows
* Molecular cloning
* Experience of AKTA FPLC use including method writing and optimisation
* Flexibility and willingness to learn new techniques and adopt new practices to support the company goals
* Accuracy, efficiency and attention to detail is essential
* Excellent communication and record-keeping skills
* Work in accordance with company Health and Safety policies, with particular emphasis on laboratory safety standards
* Experience using relevant software packages e.g. SnapGene, Benchling
Preferred Skills
* TCR & pHLA protein production
* Protein refolding
* Biophysical characterisation SPR and/or BLI
* ELISA
* Thermal shift assay