Research Associate in Evolving Quantum Mechanical Tunnelling in Enzymes
University of Manchester
Manchester, Greater Manchester, M1 7DN
£37,694 to £46,049 per annum, depending on relevant experience
Contract
NEW
Research in H‑transfer reactions involving hydride, hydrogen atom or proton transfers focuses on the role of quantum mechanical tunnelling (QMT). The project combines experimental studies of H‑tunnelling in enzyme‑catalysed reactions with directed (laboratory) evolution (DE) to explore QMT’s contribution during artificial enzyme evolution. The methodology also aims to provide a new approach to enzyme optimisation for selective deuteration in pharmaceutical synthesis and industrial biocatalysis.
Responsibilities
* Design and execute directed evolution experiments for H‑transfer enzymes using parallel selection methods.
* Measure enzyme activity, kinetics and kinetic isotope effects (KIEs).
* Perform advanced enzyme kinetics, KIE measurements and structural biology (including X‑ray crystallography).
* Collaborate with Prof. Sam Hay and Prof. Anthony Green’s research groups.
Qualifications
* Prior experience in the directed evolution of enzymes.
* Experience measuring kinetic isotope effects (KIEs) on enzyme‑catalysed reactions.
* Experience in high‑throughput chromatography highly desirable.
* Experience in one or more of the following areas would be an advantage:
o X‑ray crystallography
o Computational chemistry
What you will get in return
* Fantastic market‑leading pension scheme.
* Excellent employee health and wellbeing services, including an Employee Assistance Programme.
* Exceptional starting annual leave entitlement, plus bank holidays.
* Additional paid closure over the Christmas period.
* Local and national discounts at a range of major retailers.
Equal Opportunities
As an equal opportunities employer we welcome applicants from all sections of the community regardless of age, sex, gender (or gender identity), ethnicity, disability, sexual orientation and transgender status. All appointments are made on merit.
Hybrid working arrangements may be considered. The vacancy will close for applications at midnight on the closing date.
Contact
Name: Prof. Sam Hay
Email: Sam.Hay@manchester.ac.uk
General enquiries: Email: People.recruitment@manchester.ac.uk
Technical support: 0161 850 2004
#J-18808-Ljbffr