We are seeking
to appoint a Research Assistant to reinforce the Translational Ubiquitomics – Cancer Immunology group within the Chinese Academy of Medical Sciences (CAMS) Oxford Institute (COI), Nuffield Department of Medicine. The lab studies how cellular ubiquitylation and ISGylation influence innate immunity and cancer immune evasion, with the aim of translating these findings into valid therapeutic targets. The post will support the characterisation of the therapeutic potential of novel deISGylating enzymes using approaches spanning molecular biology, biochemistry, cellular biology, immunology and proteomics. You will be responsible for performing experiments examining deISGylating activity profiles using molecular active site probes and small molecule inhibitors; working with human and mammalian cell lines, including the generation and maintenance of knockout and knockdown cell lines using CRISPR/siRNA; preparing mass spectrometry samples from biological sources including patient tissue biopsies, cell cultures, blood and peripheral blood mononuclear cells (PBMCs); and carrying out functional validation assays such as FACS, ELISA and Incucyte. You will determine appropriate methodologies to test hypotheses, adapt experimental protocols where necessary, assist with proteomics and mass spectrometry data analysis, maintain detailed records, consolidate and present data, contribute to laboratory management and biological safety, support scientific reports and journal articles, present data at conferences, and participate in public engagement and required training activities. It is essential that you hold a degree in biology, immunology, biochemistry, chemistry, biotechnology or a similar discipline. You will have familiarity with biochemistry techniques such as immunoblotting, immunoprecipitation, pull-down assays and in vitro enzymatic activity assays, experience in handling cells and tissue culture including gene knockout/knockdown, and experience in Ubiquitomics techniques to study the ubiquitin system. Exposure to chemical biology and handling small molecule inhibitors and peptides is required, along with basic knowledge of mass spectrometry techniques and experience in sample preparation for proteomic and/or metabolomic analysis. You must demonstrate a responsible and methodical approach to laboratory work and the ability to communicate scientific results clearly and effectively.