We are seeking
to appoint a talented and ambitious Research Assistant to join a dynamic and diverse group of researchers working on bacterial pathogenesis and innate immune signalling within the Thurston Group at the Sir William Dunn School of Pathology. The post holder will be responsible for supporting the teams’ goals in understanding the molecular mechanisms of Salmonella pathogenesis. You will have the primary responsibility for supporting research projects on host pathogen interactions underpinning Salmonella virulence. You will be involved in diverse molecular and cellular work. At a molecular level, the work will involve protein expression and purification with associated in vitro assays. At a cellular level, cells infected with Salmonella will be analysed using a combination of immuno-fluorescence microscopy, flow cytometry and immunoblotting. CrispR cas9 is routinely used to create mammalian cell knockouts. RNAseq and proteomics-based approaches are also anticipated. Work will involve the preparation of mammalian cells stably expressing tagged proteins using lentiviral particles and transduction. You should hold a first degree in biochemistry, biomedical sciences, or related field. You should have some molecular laboratory experience covering techniques such as molecular cloning, DNA transfection, and protein purification. You should be able to manage your own research and administrative activities and learn techniques from laboratory members. Experience working with bacteria/microbiology, protein production and purification or immunofluorescence microscopy are desirable but not essential.