Key responsibilities
:
• Under the general leadership of the Group Leader, to assist in research aimed at understanding telomerase mechanisms
• To express and purify proteins from bacteria, insect and mammalian cell lines.
• To characterise the purified proteins and protein complexes using biochemical and biophysical
• methods.
• To maintain and transfect human cell lines
• To perform immunostaining and immunoblotting experiments
• To assist in preparing samples for cryo-EM and cryo-ET
• Keep detailed and accurate records.
• To ensure the research is carried out in accordance with good practice and in compliance with local
• policies and legal requirements.
• To plan and manage your experimental work according to the objectives set by your supervisor.
• To contribute to laboratory-wide discussions on developments within the laboratory
• To present your work at seminars within the laboratory and at external meetings.
Working relationships:
You will report to Dr Kelly Nguyen. You will interact with other members of the group, and with other scientists at the LMB as necessary.
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Education / qualifications / training required:
Essential: Bachelor’s degree in Biochemistry, Cell or Molecular Biology or a related subject.
Desirable: Research Master’s degree in a related biological subject.
Previous work experience required:
The candidate must have several of the essential skills listed below.
Essential:
• DNA construct design and cloning, including Gibson assembly, restriction cloning, bacteria transformation, and plasmid DNA isolation.
• Expression and purification of recombinant proteins from bacteria and/or human/insect cell lines using affinity, ion exchange, and/or size-exclusion chromatography.
• Experience in using automated protein purification systems.
• Biochemical characterisation of proteins: SDS-PAGE, western blotting
• Mammalian cell culture, including passaging and transfections.
Desirable:
• Working with challenging protein complexes.
• Biophysical characterisation of protein complexes (Isothermal Titration Calorimetry, Surface Plasmon Resonance, fluorescent activity assays, mass photometry, negative-stain transmission electron
microscopy)
• Knowledge of bioinformatics tools SnapGene, BLAST, ChimeraX, PyMol, ImageJ, AlphaFold
• Independence in design and optimisation of experiments relevant to the post, including
troubleshooting.
Knowledge and experience:
Essential:
• The ability to work safely without close supervision.
• The ability to follow complex protocols.
• Good communication skills to allow accurate information to be exchanged.
• Excellence in keeping lab records.
Desirable:
• Able to acquire new techniques quickly and independently.
Personal skills / behaviours / qualities:
Essential:
• Highly organised and detail-oriented individual.
• Willingness to independently develop, troubleshoot, and validate new methods or pipelines.
• A mature and conscientious attitude.
• Skills and experience in working collaboratively as a member of a team.
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