Role Overview
We are currently looking for a Research Assistant to support the Protein Structure & Biophysics department. This department is part of Discovery Science and plays a leading role in the biophysical and structural characterisation of target proteins to help with the identification of new drug targets and novel antibody therapeutics.
The Research Assistant will perform routine laboratory tasks including molecular biology, DNA preparation, mammalian cell culture and harvesting, membrane preparations, biologics sample aliquoting, inventory management and sample distribution for high throughput screening assays. The successful candidate will be exposed to a variety of discovery projects and receive regular coaching and input from the teams. Being a motivated, diligent and attentive individual, you will ensure the smooth running of laboratory workflows, work closely with other team members and use excellent communication skills to liaise with other departments.
Primary responsibilities:
• Molecular biology (cloning, transformations, bacterial cell culture), small and large-scale DNA preparation
• Small and large scale E. coli protein expression and expression analysis.
• Maintenance of mammalian cell lines, small and large scale transfections, harvesting of supernatants and cell pellets
• Maintenance of insect cells, virus generation and titres, small and large scale expression.
• Small scale protein purification and analysis
• Preparation of cell membranes
• Preparation and maintenance of stock and buffer solutions, ordering of consumables
• Protein aliquoting, maintaining accurate records of sample location using Excel and barcode scanning software
• Responding to regular sample requests, liaising with scientists to ensure timely sample delivery
Requirements
• B.Sc. in Biological Sciences or equivalent
• Understanding and/or experience in bacterial and mammalian cell culture
• Demonstrable experience in handling large numbers of samples
• Competent with pipettes (manual, electronic, single and multichannel)
• Meticulous, diligent, able to keep accurate records, good organisational skills
• Ability to communicate clearly and work efficiently in a team
• Ability to work well under pressure and respond to changing priorities to meet deadlines
• Ability to work on several projects in parallel
• Computer literate (proficiency in Microsoft Office)