We are looking for a talented and motivated postdoctoral scientist to join the lab of Professor Sir Steve Jackson ( ), based in the internationally renowned Cancer Research UK Cambridge Institute, located on the Cambridge Biomedical Campus, Cambridge, UK. Targeted radioligand therapy (TRT) is developing rapidly as a cancer treatment, yet the molecular and cellular mechanisms that shape tumour sensitivity and resistance to alpha and beta emitting TRT agents and how these mechanisms may be leveraged for therapeutic benefit are still not fully defined. This AstraZeneca CRUK collaborative project will investigate the fundamental biological determinants of responses to TRT agents, with the aim of identifying predictive biomarkers and developing rational combination strategies to enhance treatment efficacy. The postholder will:
Responsibilities
* Design and conduct CRISPR screens in human cancer cell models to identify genetic drivers of sensitivity and resistance to alpha and beta emitting TRT agents.
* Characterise how alterations in DNA damage response (DDR) pathways and associated cellular processes influence responses to TRT agents.
* Generate multiple gene knockouts, inducible expression systems, and related approaches to investigate the functional roles of candidate targets in the context of radioligand exposures.
* Carry out follow up validation and mechanistic studies, including analyses of DNA damage signalling, cell survival assays, imaging based assays, and additional CRISPR screens, to fully characterise novel genetic and functional relationships.
* Undertake specialist training and certification for work with radioactive materials.
Candidate Requirements
Candidates are expected to be comfortable with working collaboratively, intellectually independent and highly motivated and must have a PhD in biological sciences or be close to completing their postgraduate degree. Candidates should have strong experience in mammalian cell biology. A background in ionizing radiation, DNA damage/repair as well as an understanding of CRISPR-Cas9 technologies are desirable. While the project will benefit from access to expert bioinformaticians within the group, some knowledge of bioinformatics is also desirable. Applicants should have excellent written and spoken English communication skills and demonstrate excellent strong attention to detail and organisation.
You will play a key role in a large, vibrant and interactive research group, so a collaborative, friendly and collegial attitude is essential.
Benefits
The position offers a competitive salary, and the successful candidate will enjoy the diverse, vibrant and highly supportive research environment of the CRUK Cambridge Institute and the University of Cambridge.
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