Job Description
Main Purpose of the Job
The main purpose of the job is to provide support to the Beraza Lab research focused on defining the mechanisms underlying the influence of foods in the liver-gut-microbiome axis through the lifespan.
The appointed post is expected to provide expertise and support in: •Bioinformatics analysis of datasets including 10x single cell sequencing, RNA sequencing from mammalian tissues and cells •Bioinformatics analysis of datasets including 16s rRNA, shotgun and whole genome sequencing. •Design, undertake and supervise complex experimental in vivo work. •Design and perform in vitro work independently. •Competency in flow cytometry; experimental and analysis. •Competency in histopathological analysis of murine and human tissue samples including immunohistochemistry and immunofluorescence. •Competency in bright field, fluorescence and confocal microscopy. •Competency in molecular biology techniques including qPCR, western blot and ELISA. •Proved independence in data analysis, preparation and writing of research manuscripts. •Proved experience in grant applications preparation and writing. •Training and supervision of students.
Key Relationships
The Research Scientist will work closely with collaborators at QIB and the bioinformatics core team, the broader NRP (including UEA medical school and Earlham Institute) as well as other researchers across UK, Europe and Brazil. The successful candidate will actively participate in supervising undergraduate, postgraduate and PhD students.
Main Activities & Responsibilities
Bioinformatics data analysis Analysis of samples from in vivo and human studiesIn vivo studiesManuscript and grant preparationSupervision of studentsAs agreed with the line manager, any other duties commensurate with the nature of the post, for example, contributing to the work of Institute committees.
Person Profile
Education & Qualifications
RequirementPhD in biological sciences
Specialist Knowledge & Skills
RequirementBioinformatics skills to analyse 10x single cell, RNA seqBioinformatics skills to analyse 16s rRNA, shotgun and WGS In vivo work Cellular and molecular biology wet-lab techniquesFlow cytometryProficiency in R and Python for the analysis of single-cell RNA-seq, bulk RNA-seq, ATAC-seq, ChIP-seq, and metagenomic datasets.Expertise in colonic organoid culture and the complete single-cell workflow—from sample preparation to downstream analysis.Work with human tissue samples, ensuring translational relevance.
Relevant Experience
RequirementBioinformatics analysis of 10x single cell, RNA seq datasetsBioinformatics analysis of 16s rRNA, shotgun and WGS datasetsFlow cytometry; experimental and analysis. Histopathological analysis of murine and human tissue samples including immunohistochemistry and immunofluorescence.Design and perform complex in vivo experimentsData analysis, preparation and writing of research manuscriptsMolecular biology techniques including qPCR, western blot and ELISAGrant applications preparation and writingUse of brightfield, fluorescence and confocal microscopy
Interpersonal & Communication Skills
RequirementExcellent time management and organisational skillsExcellent verbal and written communication skillsAdaptabilityGood interpersonal skills, with the ability to work as part of a teamDemonstrated ability to work independently, using initiative and applying problem solving skillsA proactive approach to developing new skills and advancing interdisciplinary research
Additional Requirements
RequirementAttention to detailPromotes equality and values diversityWillingness to embrace the expected values and behaviours of all staff at the Institute, ensuring it is a great place to workAble to present a positive image of self and the Institute, promoting both the international reputation and public engagement aims of the Institute Ability to maintain confidentiality and security of information where appropriate Willingness to work outside standard working hours when required