We are seeking
a highly motivated individual with expertise in structural biology and biochemistry to join the lab of Prof Dmitry Ghilarov on a collaborative interdisciplinary project investigating how bacteria repair protein–DNA crosslinks induced by fluoroquinolone antibiotics. The project, co-supervised by Prof Stephan Uphoff, aims to understand the molecular mechanism of the bacterial exonuclease VII (Exo7) repair complex, which removes adducts of stalled type II topoisomerases from DNA and contributes to antibiotic tolerance. This research addresses fundamental questions in bacterial DNA repair and antimicrobial resistance (AMR), with the potential to inform the development of new therapeutic strategies that enhance the effectiveness of existing antibiotics. The successful candidate will have a demonstrated track record of successfully using single-particle cryo-electron microscopy to solve biological problems and have experience with protein purification and characterisation. Skills in molecular biology and genetics, and experience with DNA-binding proteins are beneficial. They will use structural and biochemical approaches to determine how Exo7 recognises and processes DNA-protein crosslinks and how this pathway contributes to bacterial survival following antibiotic treatment. This work will be tightly coordinated with live-cell fluorescent microscopy approach undertaken in parallel inthe Uphoff lab. You will be working in a modern laboratory environment for protein purification, microbiology & molecular biology. Central Oxford cryo-EM facility (COSMIC) is equipped with a 200 kV Talos Arctica screening microscope with Falcon 4i detector and a 300 kV Krios microscope with K3 detector and an energy filter. We additionally have access to a large range of outstanding facilities at the Department of Biochemistry and the wider University of Oxford including a biophysical suite, proteomics, mass photometry and native mass-spectrometry equipment. This full-time fixed-term post is funded by the BBSRC for up to 3 years in the first instance. The actual starting salary offered will be based on qualifications and relevant skills acquired and will also be determined by the funding available. About you You will hold or be near completion of a PhD/DPhil in Biochemistry, Molecular Biology, Molecular Genetics, or related discipline. You will have a passion for research and enthusiasm to work in a team on an in interdisciplinary project, with the ability to design and execute independent research projects. You have excellent communication skills, including the ability to write for publication and present research findings at group meetings and conferences. Demonstrated experience in using single-particle cryo-electron microscopy to solve biological problems and experience in protein expression, purification, and biochemical assays is essential. What We Offer Your happiness and wellbeing at work matters to us, so we offer a range of family friendly and financial benefits including: • An excellent contributory pension scheme • 38 days annual leave • A comprehensive range of childcare services • Family leave schemes • Cycle loan scheme • Membership to a variety of social and sports clubs • Discounted bus travel and Season Ticket travel loans The University also runs an enormous amount of social groups and sports clubs for those looking for more than just a great place to work. Application Process