Overview
We are looking for an enthusiastic and technically skilled full-time Research Assistant to join Dr. Maria Rostovskaya's lab at the Gurdon Institute, University of Cambridge. The successful candidate will be involved in a research project focused on the epigenetic regulation of cell fate choice during human development, supported by the MRC Career Development Fellowship.
Responsibilities
* Optimising and applying methods to genetically and epigenetically modify human pluripotent stem cell lines based on CRISPR-based techniques, and in vitro differentiation of the modified cells.
* Assist with the design, implementation and interpretation of experiments.
* Preparation of reagents, maintenance of stocks and assisting in the day-to-day running of the laboratory.
* Learn and apply a wide range of techniques including CRISPR-based genetic and epigenetic editing strategies, molecular biology (molecular cloning, RT-PCR, immunofluorescence, flow cytometry, chromatin immunoprecipitation), and advanced tissue culture (human pluripotent stem cell maintenance and in vitro differentiation, cell transfections).
Qualifications
* Laboratory experience in cell culture and molecular biology techniques are essential.
* Expertise in general areas of stem cell biology and DNA/RNA/Protein isolation/analysis would be desirable.
* The ability to work both independently and as part of a team, coupled with excellent communication, organisational and problem-solving skills is required.
Term
Fixed-term: The funds for this post are available for 2 years in the first instance.
About the lab
For more information about the laboratory please visit https://www.gurdon.cam.ac.uk/people/maria-rostovskaya/
Equality and eligibility
The University actively supports equality, diversity and inclusion and encourages applications from all sections of society. The University has a responsibility to ensure that all employees are eligible to live and work in the UK.
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