Full time. Overall purpose: To work within the group of Dr Manu Hegde at the MRC Laboratory of Molecular Biology (LMB), within a programme aimed at understanding protein biosynthesis and quality control. For cells to function properly, newly synthesised proteins must be accurately delivered to their correct cellular destinations and, in many cases, assembled with other proteins into larger complexes. Our group investigates the molecular mechanisms underlying these processes, together with the pathways that detect and resolve errors in protein maturation. In particular, we are interested in how cells recognise and clear aberrant proteins, including unassembled subunits of multi-protein complexes. Defects in these and related quality control pathways are associated with numerous human diseases, including neurodegeneration and cancer, highlighting their essential role in maintaining cellular homeostasis. Main duties/key responsibilities: • To undertake research aimed at understanding the molecular mechanisms of protein quality control. • To perform a range of support duties that may include: o Maintaining cell lines in culture o Assisting with CRISPR-Cas9 genetic screens in mammalian cells o Generating tools and reagents to support the project • To present information on research progress and outcomes within the group and the LMB. • To contribute to the smooth running of the research group, including the effective use of resources, as well as taking responsibility for communal reagents and facilities. • To contribute to the production of research reports and publications. • To plan and manage your experimental work within the project along with your supervisor and other members of the project team. • To ensure the research is carried in accordance with good practice and in compliance with local polices and legal requirements. Working relationships: You will report to Manu Hegde and Eszter Zavodszky and will interact and collaborate with Postdoctoral Scientists, Research Support staff and students, not only in your group, but also across the LMB and with external groups as necessary. Person requirements Academic qualifications: Essential: Bachelor's degree or equivalent in a relevant subject, such as biology, biochemistry, biotechnology, or genetics. Desirable: Masters degree or equivalent relevant experience. Previous work experience required: Essential: • Basic understanding of molecular and cell biology. • Experience of basic tissue culture skills/techniques (e.g. splitting cells, sterile culture technique). Desirable: • Previous experience in genetic screening. • Experimental techniques relevant to the role, which could include (but not limited to): o Flow cytometry o Western blotting o Next generation sequencing • Previous experience with bioinformatics and data analysis. Knowledge and experience: Creativity and initiative Essential: • Ability to plan and carry out experiments, collate and analyse data. • Understanding of lab health and safety. Desirable: • Experience of general lab management, including purchasing consumables and preparing communal reagents according to established protocols. • Ability to troubleshoot challenges and suggest new approaches. Independent working Essential: Proven skills in working independently and as part of a team with minimal supervision; good judgment in assessing when to ask for support. Desirable: Can independently advance existing techniques and implement new methods. Personal skills / behaviours / qualities: Essential: • Able to work collaboratively and harmoniously as part of a team. • Well organised and reliable, with exceptional record-keeping. • Flexible and able to adapt to changing priorities. Corporate/Local responsibilities & requirements You must at all times carry out your responsibilities with due regard to the: • MRC Code of Conduct • MRC Equality and Diversity policy • UKRI Health and Safety policy • UKRI Data Protection policy Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between you and your manager. The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post, the MRC and UKRI. The role holder will be required to have the appropriate level of security screening/vetting required for the role. UKRI reserves the right to run or re-run security clearance as required during the course of employment. UKRI - UK Research and Innovation UKRI is an organisation that brings together the seven disciplinary research councils, Research England and Innovate UK. Together, we build an independent organisation with a strong voice and vision ensuring the UK maintains its world-leading position in research and innovation. Supporting some of the world's most exciting and challenging research projects, we develop and operate some of the most remarkable scientific facilities in the world. We are pushing the frontiers of human knowledge through fundamental research and delivering benefits for UK society and the economy through world-class research, skills and business-led innovation. More information can be found at www.ukri.org. Choosing to come to work at UKRI means that you will have access to a whole host of benefits from a defined benefit pension scheme, excellent holiday entitlement, access to employee shopping/travel discounts and salary sacrifice cycle to work scheme. For more details, visit benefits of working for UKRI. How we support EDI in the workforce At UKRI, we believe that everyone has a right to be treated with dignity and respect, and to be provided with equal opportunities to thrive and succeed in an environment that enables them to do so. We also value diversity of thought and experience within inclusive groups, organisations and the wider community. For further information, please visit 'How we support EDI in the workforce'. Disability Confident Employer As users of the disability confident scheme, any candidate who opts into the scheme and best meets the essential criteria, will be shortlisted for interview. We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation. How to apply Online applications only preferred for this role. Please submit a CV and covering letter which clearly outlines how you fulfil the criteria specified along with your motivation for UKRI and the role. Ensure that the job reference number is included in the filename description of each document uploaded. Note that failure to address the above criteria or submit an application without a covering letter may result in the application not being considered. Assessment will only be based upon the content of your submitted covering letter and CV and not the 'experience' section of the application. UKRI seeks to ensure it creates and maintains a system of openness, fairness and inclusion - a collaborative, trusted environment, which is attractive to and accessible to everyone who is interested in developing their career with us. Artificial intelligence can be a useful tool to support your application, however, all examples and statements provided must be truthful, factually accurate and taken directly from your own experience. Where plagiarism has been identified (presenting the ideas and experiences of others, or generated by artificial intelligence, as your own) applications may be rejected and internal candidates may be subject to disciplinary action. As part of the pre-employment checks there is a requirement to undergo Baseline Personnel Security Screening. BPSS is a pre-condition of employment and failure to achieve it may mean that the employment offer is rescinded. UKRI reserves the right to run, or re-run, security clearance as required during the course of employment. The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably X-ray crystallography of proteins, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years. There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute. Our state-of-the-art laboratory provides an unsurpassed environment for both young and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation. The LMB is a great place to work and progress your career, be it in scientific research or the support functions. The LMB is a unique working environment where our researchers are rewarded by world class innovation and collaboration opportunities that the LMB name brings. The LMB is an excellent place to develop yourself further and a range of training & development opportunities will be available to you, including professional registration with the Science Council. Choosing to come to work at the LMB (part of UKRI) means that you will have access to a whole host of benefits from a defined benefit pension scheme and excellent holiday entitlement to access to employee shopping/travel discounts and salary sacrifice cycle to work scheme, as well as the chance to put the LMB and UKRI on your CV in the future. We actively support equality, diversity and inclusion in all our activities, processes and culture. We encourage applications from all sections of society irrespective of gender, race, ethnic or national origin, religion or belief, sexual orientation, disability or age. We are a disability inclusive employer and encourage disabled people to apply for this role. You are very welcome to contact us for information about the application process and any adjustments you may require; recruit@mrclmb.ac.uk. 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