An exciting opportunity has arisen for a Postdoctoral Researcher to join the Le Group at the John Innes Centre, working on cutting-edge science in the field of Molecular Microbiology.
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About the Le Group:An exciting opportunity has arisen for a Postdoctoral Researcher to join the Le Group at the John Innes Centre, working on cutting-edge science in the field of Molecular Microbiology.
Horizontal gene transfer (HGT) is a fundamental process for exchanging genetic information among bacteria. Horizontal gene transfer contributes to drive the evolution of bacterial genomes and is one of primary means for a rapid spread of antibiotic resistance and pathogenicity.
Our lab is particularly interested in horizontal gene transfer by plasmids and phage-like element. Environmental studies have showed that plasmids/phages transfer antibiotic resistance genes at extremely high frequency, which could have a major impact on antibiotic resistance gene reservoirs, hence research on this subject is of clinical and economic importance. Our lab is well-placed to answer the following important questions:
1. What is the impact of plasmids/phages on horizontal gene transfer and the evolution of bacterial genomes?
2. What are the roles of ParB-like proteins and CTPases in plasmid/phage-mediated DNA transfer/plasmid maintenance?
The role:
The practical end point of the research is to identify new targets for anti-bacterial therapies and to possibly prevent/reduce plasmid/phage-mediated antibiotic resistance.
In this role, you will also have the opportunity to:
• Shape your own project/ideas
• Engage in manuscript and proposal writing
• Use next-generation sequencing techniques (ChIP-seq, RNA-seq) and data analysis
• Use super-resolution microscopes available at the Bioimaging platform.
You will join a diverse group of students, postdoctoral researchers, and scientists and work closely with the John Innes Centre X-ray crystallography platform.
The ideal candidate:
The ideal candidate will have a PhD or equivalent experience in microbiology, particularly Pseudomonas genetics or cell biology/protein biochemistry and they will have expertise in microbiological/biological techniques and a proven ability to carry out innovative research with important, publishable outcomes. The post holder will have practical experience with gene knockout, plasmid constructions, epifluorescence microscopy and image analysis.
Good mathematical, statistical, bioinformatic and computing skills are essential, as well as a proven ability to grasp and apply new skills and concepts. They will also be able to demonstrate intellectual independence, rigor, and creativity. Practical experience in protein purification, enzymology is essential. Experience in X-ray crystallography would be advantageous but not essential.