View Vacancy Research Support Officer - Structural Studies - Dr Matteo Allegretti - LMB 2674 Open Date 01/08/2025, 08:00 Close Date 31/08/2025, 23:55 Research Institute MRC Laboratory of Molecular Biology Research Institute / Unit Information The MRC Laboratory of Molecular Biology (LMB) is one of the birthplaces of modern molecular biology and has played an important role in the development of many new techniques, most notably protein crystallography, cryo-electron microscopy, DNA sequencing and monoclonal antibodies. The LMB has a clear goal of understanding biological processes at the molecular level, with the ultimate aim of using this knowledge to tackle specific problems in human health and disease. We aim to tackle difficult, long-term problems, which often require investment over many years. There are four scientific Divisions - Structural Studies, Protein and Nucleic Acid Chemistry, Cell Biology and Neurobiology. However, scientific collaboration between the different Divisions is extensive. Scientists are well supported by excellent scientific facilities and by the LMB Operations Group which maintains the core infrastructure and services of the institute. The LMB provides an unsurpassed environment for both new and established researchers. Scientists are drawn to the LMB from all over the world, thus creating a lively and international community for the exchange of ideas and technical innovation. UK Research and Innovation is a new entity that brings together nine partners to create an independent organisation with a strong voice for research and innovation, more information can be found at www.ukri.org Band MRC - 5 Location Cambridge Salary £30,911 to £35,346 per annum Contract Type Fixed Term Job Type Science Full Time / Part Time Full Time Contract Length 2 years Job Description Overall purpose: To work with Dr Piotr Kolata, within the Group of Dr Matteo Allegretti, at the Laboratory of Molecular Biology (LMB). This is a supportive research role funded by Astra Zeneca / LMB Blue Sky collaboration, to investigate nuclear-cytoskeletal coupling in mammalian cells. The project takes an interdisciplinary approach combining single-particle electron cryo-microscopy (cryo-EM) and in-vivo electron cryo-tomography (cryo-ET), with a variety of biochemical and cell-based assays. Main duties/ Key responsibilities: • To assist in research aimed at understanding nucleo-cytoskeletal coupling. • To express and purify proteins from bacteria and insect cell lines. • To characterise the purified proteins and protein complexes using biochemical and biophysical methods. • To culture and transfect human cell lines. • To perform immunostaining and immunoblotting experiments. • To perform fluorescent microscopy of human cell lines using widefield and confocal instruments. • To assist in preparing samples for cryo-EM and cryo-ET • Keep detailed and accurate records. • To ensure the research is carried out in accordance with good practice and in compliance with local policies and legal requirements. • To plan and manage your experimental work according to the objectives set by your supervisor. • To actively develop and troubleshoot challenging assays. Working relationships: You will report to Dr Piotr Kolata and Dr Allegretti. You will interact with other members of the group, and with other scientists at the LMB as necessary. Additional information: The above lists are not exhaustive, and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post and the MRC. Person Specification Education / qualifications / training required: Essential: Bachelor's degree in Biochemistry, Cell or Molecular Biology or a related subject. Desirable: Research Master's degree in a related biological subject. Previous work experience required/ technical skills : The candidate must have several of the essential skills listed below. Essential: • DNA construct design and cloning, including Gibson assembly, restriction cloning, bacteria transformation, and plasmid DNA isolation. • Expression and purification of recombinant proteins from bacteria and/or insect cell lines using affinity, ion exchange, and/or size-exclusion chromatography. • Experience in using automated protein purification systems. • Biochemical characterisation of proteins: SDS-PAGE, western blotting • Mammalian cell culture, including passaging and transfections. • Immunofluorescence. • Light microscopy using widefield and confocal systems. Desirable: • Working with challenging protein complexes. • Biophysical characterisation of protein complexes (Isothermal Titration Calorimetry, Surface Plasmon Resonance, fluorescent activity assays, mass photometry, negative-stain transmission electron microscopy) • Knowledge of bioinformatics tools e.g. SnapGene, BLAST, ChimeraX, PyMol, ImageJ, AlphaFold • Independence in design and optimisation of experiments relevant to the post, including troubleshooting. Knowledge and experience: Essential: • The ability to work safely without close supervision. • The ability to follow complex protocols. • Good communication skills to allow accurate information to be exchanged. • Excellence in keeping lab records. Desirable: • Able to acquire new techniques quickly and independently. Personal skills / behaviours / qualities: Essential: • Highly organised and detail-oriented individual. • Willingness to independently develop, troubleshoot, and validate new methods or pipelines. • A mature and conscientious attitude. • Skills and experience in working collaboratively as a member of a team. Further Information You must at all times carry out your responsibilities with due regard to the UKRI: • Code of Conduct • Equality, Diversity and Inclusion policy • Health and Safety policy • Data Protection policy Job descriptions should be reviewed on a regular basis and at the annual appraisal. Any changes should be made and agreed between you and your manager. The above lists are not exhaustive and you are required to undertake such duties as may reasonably be requested within the scope of the post. All employees are required to act professionally, co-operatively and flexibly in line with the requirements of the post, the MRC and UKRI. The role holder will be required to have the appropriate level of security screening/vetting required for the role. UKRI reserves the right to run or re-run security clearance as required during the course of employment. The MRC is a unique working environment where scientific researchers and professional support staff can actively partake in world class innovation and collaboration opportunities and their skills and knowledge through accessing a wide catalogue of training & development, including professional registration with the Science Council. In addition, MRC (part of UKRI) provides its community of employees access to a whole host of useful benefits, including a defined benefit pension scheme and excellent holiday entitlement (30 days plus 2.5 privilege days & 8 bank holidays), family friendly policies (6 months full pay maternity & adoption leave), a range of shopping/travel discounts, access to our Employee Assistant Programme Scheme, Health and Wellbeing Support and a salary sacrifice cycle to work scheme. Please follow this link to find out more - Benefits Our success is dependent upon our ability to embrace diversity and draw on the skills, understanding and experience of all our people. We warmly invite people from diverse backgrounds and heritage, including people who identify as having a disability, to apply for a role that excites them. As "Disability Confident" employers, we guarantee to interview all applicants who have disclosed they have a disability and who meet the minimum criteria for the vacancy. UKRI supports research in areas that include animal health, agriculture and food security, and bioscience for health which includes research on animals, genetic modification and stem cell research. Whilst you may not have direct involvement in this type of research, you should consider whether this conflicts with your personal values or beliefs. We will conduct a full and comprehensive pre-employment check as an essential part of the recruitment process on all individuals that are offered a position with UKRI. This will include a security check and an extreme organisations affiliation check. The role holder will be required to have the appropriate level of security screening/vetting required for the role. UKRI reserves the right to run or re-run security clearance as required during the course of employment.